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    Structured Review

    Miltenyi Biotec catalogue no
    Catalogue No, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 280 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/catalogue+no/Human+IL-2+IS%2C+premium+grade/us12624103-678-39-37
    Average 96 stars, based on 280 article reviews
    catalogue no - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Identification of B Cell Subpopulations with Pro- and Anti-Tumorigenic Properties in an Immunocompetent Mouse Model of Head and Neck Squamous Cell Carcinoma.
    Article Snippet: Single-cell suspensions were prepared by passing tissues through a 40 μm EASY strainer (Greiner Bio-One, Frickenhausen, Germany). .. After centrifugation at 1200 rpm (=274 rcf) for 5 min, the cell pellet was resuspended in 2 mL of red cell lysis buffer (Red Blood Cell Lysis Solution (10×), Catalogue no. 130-094-183, Miltenyi Biotec, Bergisch Gladbach, Germany) and incubated at room temperature (RT) for 10 min. After the second centrifugation and removal of the supernatant, the pellet was resuspended in 5 mL of fluorescence-activated cell sorting (FACS) buffer containing 0,5% bovine serum albumin (Miltenyi, Bergisch Gladbach, Germany), 1× Dulbecco’s-Phosphate-Buffered-Saline (Gibco), and cooled at 4 ◦C. .. Tumor samples were preserved in MACS Tissue Storage Solution (Miltenyi, Bergisch Gladbach, Germany).

    Article Title: Identification of B Cell Subpopulations with Pro- and Anti-Tumorigenic Properties in an Immunocompetent Mouse Model of Head and Neck Squamous Cell Carcinoma
    Article Snippet: Single-cell suspensions were prepared by passing tissues through a 40 μm EASY strainer (Greiner Bio-One, Frickenhausen, Germany). .. After centrifugation at 1200 rpm (=274 rcf) for 5 min, the cell pellet was resuspended in 2 mL of red cell lysis buffer (Red Blood Cell Lysis Solution (10×), Catalogue no. 130-094-183, Miltenyi Biotec, Bergisch Gladbach, Germany) and incubated at room temperature (RT) for 10 min. After the second centrifugation and removal of the supernatant, the pellet was resuspended in 5 mL of fluorescence-activated cell sorting (FACS) buffer containing 0,5% bovine serum albumin (Miltenyi, Bergisch Gladbach, Germany), 1× Dulbecco’s-Phosphate-Buffered-Saline (Gibco), and cooled at 4 °C. .. Tumor samples were preserved in MACS Tissue Storage Solution (Miltenyi, Bergisch Gladbach, Germany).

    Lysis:

    Article Title: Identification of B Cell Subpopulations with Pro- and Anti-Tumorigenic Properties in an Immunocompetent Mouse Model of Head and Neck Squamous Cell Carcinoma.
    Article Snippet: Single-cell suspensions were prepared by passing tissues through a 40 μm EASY strainer (Greiner Bio-One, Frickenhausen, Germany). .. After centrifugation at 1200 rpm (=274 rcf) for 5 min, the cell pellet was resuspended in 2 mL of red cell lysis buffer (Red Blood Cell Lysis Solution (10×), Catalogue no. 130-094-183, Miltenyi Biotec, Bergisch Gladbach, Germany) and incubated at room temperature (RT) for 10 min. After the second centrifugation and removal of the supernatant, the pellet was resuspended in 5 mL of fluorescence-activated cell sorting (FACS) buffer containing 0,5% bovine serum albumin (Miltenyi, Bergisch Gladbach, Germany), 1× Dulbecco’s-Phosphate-Buffered-Saline (Gibco), and cooled at 4 ◦C. .. Tumor samples were preserved in MACS Tissue Storage Solution (Miltenyi, Bergisch Gladbach, Germany).

    Article Title: Identification of B Cell Subpopulations with Pro- and Anti-Tumorigenic Properties in an Immunocompetent Mouse Model of Head and Neck Squamous Cell Carcinoma
    Article Snippet: Single-cell suspensions were prepared by passing tissues through a 40 μm EASY strainer (Greiner Bio-One, Frickenhausen, Germany). .. After centrifugation at 1200 rpm (=274 rcf) for 5 min, the cell pellet was resuspended in 2 mL of red cell lysis buffer (Red Blood Cell Lysis Solution (10×), Catalogue no. 130-094-183, Miltenyi Biotec, Bergisch Gladbach, Germany) and incubated at room temperature (RT) for 10 min. After the second centrifugation and removal of the supernatant, the pellet was resuspended in 5 mL of fluorescence-activated cell sorting (FACS) buffer containing 0,5% bovine serum albumin (Miltenyi, Bergisch Gladbach, Germany), 1× Dulbecco’s-Phosphate-Buffered-Saline (Gibco), and cooled at 4 °C. .. Tumor samples were preserved in MACS Tissue Storage Solution (Miltenyi, Bergisch Gladbach, Germany).

    Red Blood Cell Lysis:

    Article Title: Identification of B Cell Subpopulations with Pro- and Anti-Tumorigenic Properties in an Immunocompetent Mouse Model of Head and Neck Squamous Cell Carcinoma.
    Article Snippet: Single-cell suspensions were prepared by passing tissues through a 40 μm EASY strainer (Greiner Bio-One, Frickenhausen, Germany). .. After centrifugation at 1200 rpm (=274 rcf) for 5 min, the cell pellet was resuspended in 2 mL of red cell lysis buffer (Red Blood Cell Lysis Solution (10×), Catalogue no. 130-094-183, Miltenyi Biotec, Bergisch Gladbach, Germany) and incubated at room temperature (RT) for 10 min. After the second centrifugation and removal of the supernatant, the pellet was resuspended in 5 mL of fluorescence-activated cell sorting (FACS) buffer containing 0,5% bovine serum albumin (Miltenyi, Bergisch Gladbach, Germany), 1× Dulbecco’s-Phosphate-Buffered-Saline (Gibco), and cooled at 4 ◦C. .. Tumor samples were preserved in MACS Tissue Storage Solution (Miltenyi, Bergisch Gladbach, Germany).

    Article Title: Identification of B Cell Subpopulations with Pro- and Anti-Tumorigenic Properties in an Immunocompetent Mouse Model of Head and Neck Squamous Cell Carcinoma
    Article Snippet: Single-cell suspensions were prepared by passing tissues through a 40 μm EASY strainer (Greiner Bio-One, Frickenhausen, Germany). .. After centrifugation at 1200 rpm (=274 rcf) for 5 min, the cell pellet was resuspended in 2 mL of red cell lysis buffer (Red Blood Cell Lysis Solution (10×), Catalogue no. 130-094-183, Miltenyi Biotec, Bergisch Gladbach, Germany) and incubated at room temperature (RT) for 10 min. After the second centrifugation and removal of the supernatant, the pellet was resuspended in 5 mL of fluorescence-activated cell sorting (FACS) buffer containing 0,5% bovine serum albumin (Miltenyi, Bergisch Gladbach, Germany), 1× Dulbecco’s-Phosphate-Buffered-Saline (Gibco), and cooled at 4 °C. .. Tumor samples were preserved in MACS Tissue Storage Solution (Miltenyi, Bergisch Gladbach, Germany).

    Incubation:

    Article Title: Identification of B Cell Subpopulations with Pro- and Anti-Tumorigenic Properties in an Immunocompetent Mouse Model of Head and Neck Squamous Cell Carcinoma.
    Article Snippet: Single-cell suspensions were prepared by passing tissues through a 40 μm EASY strainer (Greiner Bio-One, Frickenhausen, Germany). .. After centrifugation at 1200 rpm (=274 rcf) for 5 min, the cell pellet was resuspended in 2 mL of red cell lysis buffer (Red Blood Cell Lysis Solution (10×), Catalogue no. 130-094-183, Miltenyi Biotec, Bergisch Gladbach, Germany) and incubated at room temperature (RT) for 10 min. After the second centrifugation and removal of the supernatant, the pellet was resuspended in 5 mL of fluorescence-activated cell sorting (FACS) buffer containing 0,5% bovine serum albumin (Miltenyi, Bergisch Gladbach, Germany), 1× Dulbecco’s-Phosphate-Buffered-Saline (Gibco), and cooled at 4 ◦C. .. Tumor samples were preserved in MACS Tissue Storage Solution (Miltenyi, Bergisch Gladbach, Germany).

    Article Title: Identification of B Cell Subpopulations with Pro- and Anti-Tumorigenic Properties in an Immunocompetent Mouse Model of Head and Neck Squamous Cell Carcinoma
    Article Snippet: Single-cell suspensions were prepared by passing tissues through a 40 μm EASY strainer (Greiner Bio-One, Frickenhausen, Germany). .. After centrifugation at 1200 rpm (=274 rcf) for 5 min, the cell pellet was resuspended in 2 mL of red cell lysis buffer (Red Blood Cell Lysis Solution (10×), Catalogue no. 130-094-183, Miltenyi Biotec, Bergisch Gladbach, Germany) and incubated at room temperature (RT) for 10 min. After the second centrifugation and removal of the supernatant, the pellet was resuspended in 5 mL of fluorescence-activated cell sorting (FACS) buffer containing 0,5% bovine serum albumin (Miltenyi, Bergisch Gladbach, Germany), 1× Dulbecco’s-Phosphate-Buffered-Saline (Gibco), and cooled at 4 °C. .. Tumor samples were preserved in MACS Tissue Storage Solution (Miltenyi, Bergisch Gladbach, Germany).

    Fluorescence:

    Article Title: Identification of B Cell Subpopulations with Pro- and Anti-Tumorigenic Properties in an Immunocompetent Mouse Model of Head and Neck Squamous Cell Carcinoma.
    Article Snippet: Single-cell suspensions were prepared by passing tissues through a 40 μm EASY strainer (Greiner Bio-One, Frickenhausen, Germany). .. After centrifugation at 1200 rpm (=274 rcf) for 5 min, the cell pellet was resuspended in 2 mL of red cell lysis buffer (Red Blood Cell Lysis Solution (10×), Catalogue no. 130-094-183, Miltenyi Biotec, Bergisch Gladbach, Germany) and incubated at room temperature (RT) for 10 min. After the second centrifugation and removal of the supernatant, the pellet was resuspended in 5 mL of fluorescence-activated cell sorting (FACS) buffer containing 0,5% bovine serum albumin (Miltenyi, Bergisch Gladbach, Germany), 1× Dulbecco’s-Phosphate-Buffered-Saline (Gibco), and cooled at 4 ◦C. .. Tumor samples were preserved in MACS Tissue Storage Solution (Miltenyi, Bergisch Gladbach, Germany).

    Article Title: Identification of B Cell Subpopulations with Pro- and Anti-Tumorigenic Properties in an Immunocompetent Mouse Model of Head and Neck Squamous Cell Carcinoma
    Article Snippet: Single-cell suspensions were prepared by passing tissues through a 40 μm EASY strainer (Greiner Bio-One, Frickenhausen, Germany). .. After centrifugation at 1200 rpm (=274 rcf) for 5 min, the cell pellet was resuspended in 2 mL of red cell lysis buffer (Red Blood Cell Lysis Solution (10×), Catalogue no. 130-094-183, Miltenyi Biotec, Bergisch Gladbach, Germany) and incubated at room temperature (RT) for 10 min. After the second centrifugation and removal of the supernatant, the pellet was resuspended in 5 mL of fluorescence-activated cell sorting (FACS) buffer containing 0,5% bovine serum albumin (Miltenyi, Bergisch Gladbach, Germany), 1× Dulbecco’s-Phosphate-Buffered-Saline (Gibco), and cooled at 4 °C. .. Tumor samples were preserved in MACS Tissue Storage Solution (Miltenyi, Bergisch Gladbach, Germany).

    FACS:

    Article Title: Identification of B Cell Subpopulations with Pro- and Anti-Tumorigenic Properties in an Immunocompetent Mouse Model of Head and Neck Squamous Cell Carcinoma.
    Article Snippet: Single-cell suspensions were prepared by passing tissues through a 40 μm EASY strainer (Greiner Bio-One, Frickenhausen, Germany). .. After centrifugation at 1200 rpm (=274 rcf) for 5 min, the cell pellet was resuspended in 2 mL of red cell lysis buffer (Red Blood Cell Lysis Solution (10×), Catalogue no. 130-094-183, Miltenyi Biotec, Bergisch Gladbach, Germany) and incubated at room temperature (RT) for 10 min. After the second centrifugation and removal of the supernatant, the pellet was resuspended in 5 mL of fluorescence-activated cell sorting (FACS) buffer containing 0,5% bovine serum albumin (Miltenyi, Bergisch Gladbach, Germany), 1× Dulbecco’s-Phosphate-Buffered-Saline (Gibco), and cooled at 4 ◦C. .. Tumor samples were preserved in MACS Tissue Storage Solution (Miltenyi, Bergisch Gladbach, Germany).

    Article Title: Identification of B Cell Subpopulations with Pro- and Anti-Tumorigenic Properties in an Immunocompetent Mouse Model of Head and Neck Squamous Cell Carcinoma
    Article Snippet: Single-cell suspensions were prepared by passing tissues through a 40 μm EASY strainer (Greiner Bio-One, Frickenhausen, Germany). .. After centrifugation at 1200 rpm (=274 rcf) for 5 min, the cell pellet was resuspended in 2 mL of red cell lysis buffer (Red Blood Cell Lysis Solution (10×), Catalogue no. 130-094-183, Miltenyi Biotec, Bergisch Gladbach, Germany) and incubated at room temperature (RT) for 10 min. After the second centrifugation and removal of the supernatant, the pellet was resuspended in 5 mL of fluorescence-activated cell sorting (FACS) buffer containing 0,5% bovine serum albumin (Miltenyi, Bergisch Gladbach, Germany), 1× Dulbecco’s-Phosphate-Buffered-Saline (Gibco), and cooled at 4 °C. .. Tumor samples were preserved in MACS Tissue Storage Solution (Miltenyi, Bergisch Gladbach, Germany).

    Purification:

    Article Title: Antibodies to Cryptic Epitopes in Distant Homologues Underpin a Mechanism of Heterologous Immunity between Plasmodium vivax PvDBP and Plasmodium falciparum VAR2CSA
    Article Snippet: Parasites were regularly selected for adhesion to CSA (catalogue no. C9819; Sigma-Aldrich) to enrich for parasites expressing VAR2CSA. .. Mature parasites were magnetically purified using the VarioMACS separator according to the manufacturer’s instructions (LD columns, catalogue no. 130-042-901; Miltenyi Biotec). .. Inhibition of binding assay (IBA).

    Article Title: Antibodies to Cryptic Epitopes in Distant Homologues Underpin a Mechanism of Heterologous Immunity between Plasmodium vivax PvDBP and Plasmodium falciparum VAR2CSA
    Article Snippet: Parasites were regularly selected for adhesion to CSA (catalogue no. C9819; Sigma-Aldrich) to enrich for parasites expressing VAR2CSA. .. Mature parasites were magnetically purified using the VarioMACS separator according to the manufacturer’s instructions (LD columns, catalogue no. 130-042-901; Miltenyi Biotec). .. Ten spots were drawn in a semicircle around the outer edge of the bottom of a Petri dish (catalogue no. 351029; Corning).

    Article Title: A conserved epitope in VAR2CSA is targeted by a cross-reactive antibody originating from Plasmodium vivax Duffy binding protein.
    Article Snippet: .. IEs containing mature parasites were purified using the VarioMACS separator according to the manufacturer’s instructions (LD columns, catalogue no. 130-042-901; Miltenyi Biotec). ..

    Article Title: A conserved epitope in VAR2CSA is targeted by a cross-reactive antibody originating from Plasmodium vivax Duffy binding protein
    Article Snippet: .. IEs containing mature parasites were purified using the VarioMACS separator according to the manufacturer’s instructions (LD columns, catalogue no. 130-042-901; Miltenyi Biotec). ..

    Activation Assay:

    Article Title: Engineered antibodies that bind LAG3
    Article Snippet: .. SEB activation: Fresh cynomolgus monkey peripheral blood mononuclear cells (cyno-PBMCs) were isolated and plated at 2.0E+05 cells/well in a U-bottom plate in 200 μL of complete RPMI (RPMI 1640+10% FCS+Pen/Strep) containing recombinant human IL-2 at 200 U/mL (Miltenyi Biotec; catalogue no. 130-097-748) with or without 300 ng/mL of Staphylococcal Enterotoxin B (SEB) from Toxin Technology, Inc. (catalogue No. 122, lot. ..

    Isolation:

    Article Title: Engineered antibodies that bind LAG3
    Article Snippet: .. SEB activation: Fresh cynomolgus monkey peripheral blood mononuclear cells (cyno-PBMCs) were isolated and plated at 2.0E+05 cells/well in a U-bottom plate in 200 μL of complete RPMI (RPMI 1640+10% FCS+Pen/Strep) containing recombinant human IL-2 at 200 U/mL (Miltenyi Biotec; catalogue no. 130-097-748) with or without 300 ng/mL of Staphylococcal Enterotoxin B (SEB) from Toxin Technology, Inc. (catalogue No. 122, lot. ..

    Recombinant:

    Article Title: Engineered antibodies that bind LAG3
    Article Snippet: .. SEB activation: Fresh cynomolgus monkey peripheral blood mononuclear cells (cyno-PBMCs) were isolated and plated at 2.0E+05 cells/well in a U-bottom plate in 200 μL of complete RPMI (RPMI 1640+10% FCS+Pen/Strep) containing recombinant human IL-2 at 200 U/mL (Miltenyi Biotec; catalogue no. 130-097-748) with or without 300 ng/mL of Staphylococcal Enterotoxin B (SEB) from Toxin Technology, Inc. (catalogue No. 122, lot. ..



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    Image Search Results


    Synthetic Accessibility scores (SA scores) for each generation in the reconstruction paths of Erdafitinib generated by ChemBang, starting from three different molecular seeds: (A) pyrazole, (B) benzene, and (C) quinoxaline. The dashed gray line indicates a stricter SA score reference of 4.5, while the commonly suggested threshold by the original SA score authors is 6. “Bio space” refers to molecules from ChEMBL33 and SureChEMBL; “Chem space” includes in‐stock compound catalogues used in this study; and the “ER diversity set” corresponds to the Enamine REAL diversity set.

    Journal: Molecular Informatics

    Article Title: ChemBang: Expanding the Chemical Space Around Small Molecules

    doi: 10.1002/minf.70036

    Figure Lengend Snippet: Synthetic Accessibility scores (SA scores) for each generation in the reconstruction paths of Erdafitinib generated by ChemBang, starting from three different molecular seeds: (A) pyrazole, (B) benzene, and (C) quinoxaline. The dashed gray line indicates a stricter SA score reference of 4.5, while the commonly suggested threshold by the original SA score authors is 6. “Bio space” refers to molecules from ChEMBL33 and SureChEMBL; “Chem space” includes in‐stock compound catalogues used in this study; and the “ER diversity set” corresponds to the Enamine REAL diversity set.

    Article Snippet: A diverse dataset of unique molecular structures was assembled from eight in‐stock chemical catalogues—ChemDiv, ChemSpace, LifeChemicals, Mcule, Vitas, WixilabNetwork, Akos, and Zinc20 [ ]—as well as the curated bioactivity database ChEMBL33 [ ].

    Techniques: Generated